Figure 2.
The presence of PDCs enhances CpG-stimulated cytokine production in naive and memory B cells. Total B cells, naive B cells, or memory B cells were incubated alone (□ 1 × 105 B cells/200 μL/well) or in the presence of PDCs (▪ 1 × 104 PDCs/200 μL/well) and were stimulated with CpG-C (3 μg/mL), anti-Ig (10 μg/mL), or a combination of CpG-C and anti-Ig. After 72 hours, IL-6 (A), TNF-α (B), or IL-10 (C) was measured in the supernatants by ELISA. Data are shown as means ± SEM of 4 (IL-6 and TNF-α) or of 2 (IL-10) independent experiments.
