Figure 3.
PDCs contribute to B-cell activation by soluble factors. A transwell system was used to separate PDCs and B cells. B cells were cultured in the lower compartment of transwells (5 × 105 B cells/well) and PDCs in the upper compartment (5 × 104 PDC; ▨). B cells and PDCs were also cocultured without transwell (▪), and B cells were cultured without PDC (□). Cells were stimulated with CpG-C (3 μg/mL) and anti-Ig (10 μg/mL). After 3 days the MFI of CD86 was analyzed (A), and IL-6 (B) and IL-10 (C) were measured in the supernatants by ELISA. The means of 3 independent experiments ± SEM are shown.
