Figure 5.
The expression of TLR9 mRNA in human B cells is independently down-regulated by CpG and antigen. B cells (1 × 106/mL/well in 24-well plates) were stimulated with CpG-B (3 μg/mL), anti-Ig (10 μg/mL), and recombinant IFN-α (1000 IU/mL, ▨) for 12 hours. Expression of TLR9 and TLR7 mRNA was determined by quantitative real-time PCR and is depicted as the number of transcripts per 103 copies of the housekeeping gene cyclophilin-B (CPB). The means ± SEM of 3 independent experiments are shown. P < .05
