Figure 5.
![Figure 5. Increased [Ca2+]i correlated with mast cell degranulation rather than survival. (A) FcRγ-deficient BMMCs expressing CD8/γ were loaded with indo-1 and stimulated with graded amounts of anti-CD8. The increase of [Ca2+]i was determined and expressed as described in “Materials and methods.” Bars on the right side represent cell survival (white bar) and β-hex release (black bar) at the same mAb concentrations. (B) Normal BMMCs were also stimulated with 10 μg/mL anti-DNP IgE (top graph) or a graded amount of DNP-HSA after sensitization with 1 μg/mL anti-DNP IgE for 16 hours at 37°C.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/103/8/10.1182_blood-2003-08-2944/6/zh80080459910005.jpeg?Expires=1735843486&Signature=mleC1-0mB9DXKKRewuFT2q6e1-0r6M0L~7f~WSK-wlrkR-0YiYNZZHiWry774plnGRFS9yvNzKuoAA1n~DHhiBmE705OREiJay3GL70dRMg~GNlkau7nbgjFPsWlOLyW2X~lorglavex2P4R-l68vVnpVG4dxUwMkNl0zuYsVT5T57JIw9vzjgN~LWmEPFMf567~8Ymub0P2o3UEdXtXt6ONkHo4QsnC81A7kmjU4V8zpXMEDRUdq9HRXce~gUVFnkc2R5KU858WARl6l7rUZexmaw74vxgWejtDibzaaaJAYdQFPmK3IEVkhlZgeM20vFv1kD0y2v7mZoy02i-Ipg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Increased [Ca2+]i correlated with mast cell degranulation rather than survival. (A) FcRγ-deficient BMMCs expressing CD8/γ were loaded with indo-1 and stimulated with graded amounts of anti-CD8. The increase of [Ca2+]i was determined and expressed as described in “Materials and methods.” Bars on the right side represent cell survival (white bar) and β-hex release (black bar) at the same mAb concentrations. (B) Normal BMMCs were also stimulated with 10 μg/mL anti-DNP IgE (top graph) or a graded amount of DNP-HSA after sensitization with 1 μg/mL anti-DNP IgE for 16 hours at 37°C.
