American Society of Hematology

Figure 6.

$Figure 6. Constitutive raft localization is not sufficient for BMMC survival. (A) Schematic representation of LAT/γ and LAT(CA)/γ chimera. (B) Subcellular localization of LAT/γ. FcRγ-deficient BMMCs expressing pMX-IRES-GFP vector only (Mock), LAT/γ, or LAT(CA)/γ were subjected to density gradient centrifugation as previously described. Each fraction was blotted with anti-FcRγ (left) and anti-Lyn (right) as a control. (C) Cell survival after IL-3 depletion. pMX-IRES-GFP (Mock)–, LAT/γ-, and LAT(CA)/γ-infected bulk BMMCs were cultured in the absence of IL-3, and the percentage of viable cells at the indicated days was examined. The percentages of bicistronic GFP-positive population were around 30% in each line. Data were expressed as the percentage of viable cell numbers at day 0 for the GFP-negative (uninfected internal control) and GFP-positive (infected) gated population.$

Constitutive raft localization is not sufficient for BMMC survival. (A) Schematic representation of LAT/γ and LAT(CA)/γ chimera. (B) Subcellular localization of LAT/γ. FcRγ-deficient BMMCs expressing pMX-IRES-GFP vector only (Mock), LAT/γ, or LAT(CA)/γ were subjected to density gradient centrifugation as previously described. Each fraction was blotted with anti-FcRγ (left) and anti-Lyn (right) as a control. (C) Cell survival after IL-3 depletion. pMX-IRES-GFP (Mock)–, LAT/γ-, and LAT(CA)/γ-infected bulk BMMCs were cultured in the absence of IL-3, and the percentage of viable cells at the indicated days was examined. The percentages of bicistronic GFP-positive population were around 30% in each line. Data were expressed as the percentage of viable cell numbers at day 0 for the GFP-negative (uninfected internal control) and GFP-positive (infected) gated population.

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