Monomeric CD8/γ failed to induce degranulation but not survival upon anti-CD8 treatment. (A) Schematic representation of CD8/γ and CD8(CS)/γ. (B) Lack of dimer formation in CD8(CS)/γ. The 2B4 T-cell hybridomas were infected with vector alone (Mock), CD8/γ, or CD8(CS)/γ. Cells were lysed and analyzed by SDS-PAGE in the presence (reducing) and absence (nonreducing) of 2.5 μM 2-mercaptoethanol in the sample buffer. The membrane was blotted with anti-FcRγ. (C) Cell survival after IL-3 depletion. BMMCs expressing truncated CD8 (CD8), CD8/γ, and CD8(CS)/γ were sorted and cultured in the presence of different concentrations of anti-CD8 after IL-3 depletion. Percentage of viable cells at the indicated days is shown. (D) Histamine release upon anti-CD8 stimulation. Each cell line was stimulated using the same conditions as for panel C for 30 minutes. Histamine release into the culture supernatant was determined by ELISA. N.D. indicates not detected. Data were means ± SDs of triplicate assays.