Cytochemistry and phenotype of cells obtained from 7-day culture of freshly isolated CD14⁺ cells with M-CSF. (A) Photographs of May-Grünwald-Giemsa–, MPO-, and double specific/nonspecific esterase–stained cytospin preparations; original magnification, × 400. (B) The expression of CD15/CD14, MPO, M-CSFR, lactoferrin, mannose receptor, and HLA-DR was analyzed using a FACSCalibur flow cytometer. In the histograms, the thick and thin lines show the expression of the indicated molecules and isotype controls, respectively. Yield of cultured cells was 52.8% ± 9.8% (n = 5). Representative data from 5 independent experiments are shown.