Cytochemistry and phenotype of cells obtained from culture of freshly isolated CD15⁺CD14^- neutrophils with GM-CSF, TNF-α, IFN-γ, and IL-4 for 11 days. (A) Expression pattern of CD15/CD14. (B) The expression of CD15/CD14, MPO, lactoferrin, M-CSFR, mannose receptor, and HLA-DR was analyzed using a FACSCalibur flow cytometer. In the histograms, the thick and thin lines show the expression of the indicated molecules and isotype controls, respectively. Representative data from 5 independent experiments are shown.