Figure 5.
Expression profiles of neutrophil- and macrophage-specific genes. (A) Hierarchical clustering based on the expression intensities in CD15+CD14- neutrophils, CD15+CD14- neutrophil–derived macrophages, and CD14+ cell–derived macrophages was conducted for 10 genes with a specific expression in CD15+CD14- neutrophils and CD14+ cell–derived macrophages (top panel) or in CD14+ cell–derived macrophages (bottom panel). Each row represents a single gene on the microarray, and each column represents a separate sample. Expression intensity of each gene is shown color coded, according to the scale at the bottom, and the gene symbols are indicated on the right. Expression data of these genes are available on request. (B) The gene tree was constructed using 2-way clustering analysis of the genes that are differentially (Welch ANOVA, P < .001) expressed between CD15+CD14- neutrophils and CD14+ cell–derived macrophages. Each row represents a single gene on the microarray, and each column represents a separate sample. Expression intensity of each gene is shown color coded, according to the scale in panel A.