Phagocytic assay of CD15⁺CD14^- neutrophil–derived macrophages with FITC-dextran and FITC-latex beads. (A) CD15⁺CD14^- neutrophil– and CD14⁺ cell–derived macrophages were incubated with FITC-dextran for 1 hour at 37°C or 4°C, washed with cold PBS supplemented with 1% FBS, and analyzed using a FACSCalibur flow cytometer. Data are presented using histograms. (B) CD15⁺CD14^- neutrophil– and CD14⁺ cell–derived macrophages were incubated with FITC-latex beads for 1 hour at 37°C or 4°C, washed with cold PBS supplemented with 1% FBS, and analyzed with a FACSCalibur flow cytometer. Data are expressed as percentage of positive cells. Experiments were repeated 5 times with identical results.