Overexpression of γ-catenin in Sca1⁺/lin- HSCs. (A) Differentiation of HSCs transduced with γ-catenin compared with that of PML/RARα-expressing HSCs in absence/presence of G/GM-CSF. Gr-1 and Mac-1 were detected as markers of myeloid differentiation and Sca1 and c-kit were detected as stem markers. One representative experiment of at least 3 performed is given. (B) Effect of γ-catenin and X-RARα on cell cycle progression of Sca1+/lin- HSCs. Cell cycle was analyzed 36 hours after transduction by staining the cells with propidium iodide (PI) or by BrdU incorporation (BrdU). For each assay one representative experiment of 3 is given.