Colony formation of Sca1⁺/lin- HSCs expressing γ-catenin. (A) Replating efficiency of Sca1+/lin- stem cells transduced with γ-catenin, PML/RARα,or PLZF/RARα (Roman numerals indicate the number of the plating round). Mock-transduced cells were used as control. The cells were seeded in semisolid medium. At day 10 the cells were harvested, analyzed, and replated. (B) Morphology of the colonies at the plating IV. Original magnification, × 25. (C) Differentiation-specific surface marker expression. Gr-1 and Mac-1 are markers for myeloid differentiation and Sca1 and c-kit are stem cell markers. (D) Morphologic analysis of cells stained with May-Grünwald-Giemsa. Original magnification, × 400. (E) Differentiation of the γ-catenin-expressing cells at the plating IV in absence/presence of G/GM-CSF. One representative of at least 3 experiments is shown.