Figure 7.
Figure 7. Leukemogenic potential of Sca1+/lin- transduced with γ-catenin and inoculated into sublethally irradiated mice. (A-C) Analysis of the mouse that developed disease symptoms after 9 months. (A) Western blot analysis of γ-catenin expression in the MNCs isolated from the BM and the spleen. Equal protein loading was confirmed after stripping of the membrane and staining with an anti-β-tubulin antibody. (B) Morphology of BM and spleen cells stained with May-Grünwald Giemsa. Two magnifications are given (× 400 and × 600). (C) Immunologic characterization of BM and spleen cells. Gr-1and Mac-1 characterize the myeloid lineage, B220 the B-lymphocytic lineage, CD3ϵ the T-lymphocytic lineage, and Ter119 the erythroid lineage. (D-F) Analysis of the mouse that developed disease symptoms after 12 months. (D) Western blot analysis of γ-catenin expression in the MNCs isolated from the BM and the spleen. Equal protein loading was confirmed by Ponceau staining of the membrane. (E) Morphology of BM and spleen cells stained with May-Grünwald Giemsa. Original magnification × 400. (F) Immunologic characterization of BM and spleen cells distinguishing between GFP-positive and -negative population. Gr-1 and Mac-1 characterize the myeloid lineage, B220 the B-lymphocytic lineage, CD3ϵ the T-lymphocytic lineage, and Ter119 the erythroid lineage.

Leukemogenic potential of Sca1+/lin-transduced with γ-catenin and inoculated into sublethally irradiated mice. (A-C) Analysis of the mouse that developed disease symptoms after 9 months. (A) Western blot analysis of γ-catenin expression in the MNCs isolated from the BM and the spleen. Equal protein loading was confirmed after stripping of the membrane and staining with an anti-β-tubulin antibody. (B) Morphology of BM and spleen cells stained with May-Grünwald Giemsa. Two magnifications are given (× 400 and × 600). (C) Immunologic characterization of BM and spleen cells. Gr-1and Mac-1 characterize the myeloid lineage, B220 the B-lymphocytic lineage, CD3ϵ the T-lymphocytic lineage, and Ter119 the erythroid lineage. (D-F) Analysis of the mouse that developed disease symptoms after 12 months. (D) Western blot analysis of γ-catenin expression in the MNCs isolated from the BM and the spleen. Equal protein loading was confirmed by Ponceau staining of the membrane. (E) Morphology of BM and spleen cells stained with May-Grünwald Giemsa. Original magnification × 400. (F) Immunologic characterization of BM and spleen cells distinguishing between GFP-positive and -negative population. Gr-1 and Mac-1 characterize the myeloid lineage, B220 the B-lymphocytic lineage, CD3ϵ the T-lymphocytic lineage, and Ter119 the erythroid lineage.

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