Figure 3.
![Figure 3. Mutation analysis of β/SMMHC for the interaction with the Runt domain. (A) Identification of the second binding domain in β/SMMHC. GST-tagged Runt domain (GST-RD[25-190]) was mixed with various deletion constructs of β/SMMHC, and GST pull-down experiment was performed. Lanes 1 to 12 indicate 10% of the input of in vitro– translated β/SMMHC or its derivatives; lanes 13 to 24, control pull-down experiment with GST alone; and lanes 25 to 36, GST pull-down experiment using GST-RD(25-190). (B) The schematic diagram of β/SMMHC-derived constructs and the result of the GST pull-down experiments. The binding activities of each construct are listed to the right. X marks in panel B indicate mutations.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/103/8/10.1182_blood-2003-07-2188/6/zh80080459760003.jpeg?Expires=1735548260&Signature=JnuG32lQbsKDVF9AVMqe26QDGzWmtQ4vuvuoI8UkH0XDdrBoJN3fXrXQWWGszZFsA7jtJlunTPL5gQY0UxKbdLN~zzghboVOLQ~x~Fbtu-zH-BGW6OSJGNB9R6M1vmZNUjZ-MBu6osvdhhEgoCBTos-51h~oipT6B3N0PJ4-lCxgKO7PQvpXJfjzN-VtbfZV2Qg-qxtPHLVMvldDOabx~WatVM34iMxj7SX64WyRAFoVTHOwE1wAbPWS7lFvGJlqPWvE-Nt1Cnq8Huzdxr2CB319Mmqo9wKlxmt-tAzvPZQs0EoVY55qZ43bg3GF6jqruF7IGQmqFUrKCYfsoHSrjQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Mutation analysis of β/SMMHC for the interaction with the Runt domain. (A) Identification of the second binding domain in β/SMMHC. GST-tagged Runt domain (GST-RD[25-190]) was mixed with various deletion constructs of β/SMMHC, and GST pull-down experiment was performed. Lanes 1 to 12 indicate 10% of the input of in vitro– translated β/SMMHC or its derivatives; lanes 13 to 24, control pull-down experiment with GST alone; and lanes 25 to 36, GST pull-down experiment using GST-RD(25-190). (B) The schematic diagram of β/SMMHC-derived constructs and the result of the GST pull-down experiments. The binding activities of each construct are listed to the right. X marks in panel B indicate mutations.
