The effect of the PI3Kδ inhibition or deletion on neutrophil transmigration. (A) Representative micrographs depicting LTB4-induced transmigration of neutrophils in a WT mouse pretreated with IC87114 (i), an animal deficient in p110δ (ii), or WT animal pretreated with vehicle control (iii). Each micrograph represents an individual experiment one hour after continuous exposure to LTB4 as described in “Materials and methods.” (B) Chemoattractant dose response curve for murine neutrophil transwell migration to LTB4 in the absence or presence of DMSO (0.3%). (C) Neutrophil chemotaxis at a concentration of chemoattractant that supports half maximal transmigration (30 nM). Results are from 4 experiments performed in triplicate (mean ± SD). *P < .01 versus control.
