Figure 7.
![Figure 7. The impact of p110δ inhibition or deletion on PIP3 production in stimulated neutrophils. (A) Thin-layer chromatography of phospholipid extracts from neutrophils purified from WT or p110δ-deficient animals. Cells labeled with [32P] were pretreated with IC87114 (10 μM) or vehicle control prior to activation with fMLP (1 μM). PIP3 standard is shown for comparison. (B) PIP3 quantitation. Results are expressed as a percentage of total phospholipids and represent 2 independent experiments performed in duplicate.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/103/9/10.1182_blood-2003-05-1667/6/zh80090460640007.jpeg?Expires=1736048173&Signature=AQWu54fxLIBcUA4RyyIyyS~xdWk9oO3UL6U64Dh6VjzEoZo~4kWFJHpeel4oTLnOupscnKQMZjFaT4TACwYUSQPAr-lnuHzVGSA1T9tR4rrVOlYpQR5qh3GLEsgfvDEOnM0QEsGPYQVMpht5o6rZb4Kc~b4MQU80wB-eiTV5AaYos0FbPAfZ38iCYISpnoYDrV1yx9F1-1-mm-xvF2OgLyApqr4Mp4y5cHmW9kX9SBDOr6W4RoVJAZlUCX37UDOhslaFvQmq7h4JSBOgSEPfpaHq7dgvL6tWxQnHxdY~de90VYb7mTgbu72W8KxrOU358JYF14lxwadVB2UNgOqCng__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The impact of p110δ inhibition or deletion on PIP3 production in stimulated neutrophils. (A) Thin-layer chromatography of phospholipid extracts from neutrophils purified from WT or p110δ-deficient animals. Cells labeled with [32P] were pretreated with IC87114 (10 μM) or vehicle control prior to activation with fMLP (1 μM). PIP3 standard is shown for comparison. (B) PIP3 quantitation. Results are expressed as a percentage of total phospholipids and represent 2 independent experiments performed in duplicate.
