American Society of Hematology

Figure 5.

$Figure 5. IL-2 dose influences cytotoxicity and DAP10 expression but has no effect on NKG2D cell surface expression. Cells were isolated from donors as described in “Materials and methods” and divided into 2 separate fractions that were treated identically except that one received high doses of IL-2 (300 U/mL) and the other low-dose IL-2 (30 U/mL). (A) FACS analysis shows no difference in NKG2D expression on CD3+CD8+ cells at IL-2 doses of either 30 or 300 U/mL. Dotted histograms represent isotype control antibodies and solid lines NKG2D. (B) Cytolysis against 721-221 cells (E/T = 10:1) demonstrates no cytolysis of targets when cells were cultured with 30 U/mL IL-2, but significant cytolysis when cultured in 300 U/mL. (C) Expression of DAP10 by RT-PCR was detected in cells that were cultured in high (300 U/mL) but not low doses of IL-2 (30 U/mL). Results are representative of 3 individual experiments.$

IL-2 dose influences cytotoxicity and DAP10 expression but has no effect on NKG2D cell surface expression. Cells were isolated from donors as described in “Materials and methods” and divided into 2 separate fractions that were treated identically except that one received high doses of IL-2 (300 U/mL) and the other low-dose IL-2 (30 U/mL). (A) FACS analysis shows no difference in NKG2D expression on CD3⁺CD8⁺ cells at IL-2 doses of either 30 or 300 U/mL. Dotted histograms represent isotype control antibodies and solid lines NKG2D. (B) Cytolysis against 721-221 cells (E/T = 10:1) demonstrates no cytolysis of targets when cells were cultured with 30 U/mL IL-2, but significant cytolysis when cultured in 300 U/mL. (C) Expression of DAP10 by RT-PCR was detected in cells that were cultured in high (300 U/mL) but not low doses of IL-2 (30 U/mL). Results are representative of 3 individual experiments.

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