Regulation of Bcl-2 family antiapoptotic proteins by BAFF and APRIL. (A) XG-13, XG-14, and RPMI8226 cells were starved overnight before culture with no cytokine, or with IL-6 (30 ng/mL), BAFF (800 ng/mL), or APRIL (800 ng/mL) for 6 hours in RPMI–1% BSA. At the end of the culture, cells were immediately lysed and assayed for Bcl-2 family antiapoptotic protein expression using Western blot analysis. In this experiment, STAT3 expression was used as loading protein control. (B) Blots of 3 independent experiments were scanned and the values were normalized using STAT3-band intensities as internal standards. Results are the median values for the 3 main Bcl-2 family members expressed in the XG-13 HMCLs starved of IL-6 and cultured for 6 hours with no cytokine, or with IL-6 (30 ng/mL), BAFF (800 ng/mL), or APRIL (800 ng/mL). ^*Significant increase in expression with a Student t test for pairs (P < .05).