Figure 2.
Treatment of human CD34+ progenitor cells with anti-CD44 mAb or soluble HA inhibits the engraftment of SCID repopulating cells. Enriched CB CD34+ cells were preincubated with antihuman CD44 mAb F10-44-2 or with HA and transplanted (2 × 105 cells per mouse) into sublethally irradiated NOD/SCID mice. The percentage of human cells in cell suspension prepared from the murine BM and spleen was determined 6 weeks after transplantation by flow cytometry using human specific anti-CD45–FITC/CD19-PE mAbs. Ctrl indicates untreated cells. Data represent average of 3 experiments ± SD (3 mice per treatment in each experiment, *P < .01) (A). (B) A representative flow cytometry analysis is shown, and percentage of human cells is indicated.
