Effect of FVIIa and other agonists on the induction of IL-8 mRNA. Quiescent monolayers of MDA-MB-231 cells were treated with FVIIa (50 nM), thrombin (10 nM), plasmin (50 nM), trypsin (50 nM), FXa (50 nM), APC (50 nM), PAR-2–specific peptide agonist SLIGKV (25 μM) and PAR-1–specific peptide agonist TFLLRN (25 μM) for 75 minutes. Total RNA was analyzed for IL-8 expression by Northern blot analysis. (A) Representative autoradiograph. (B) Quantitative data (mean ± SEM, n = 4 to 7). ^*Value significantly higher (P < .05) than the control value.