Effect of FXa on FVIIa-induced IL-8 expression. MDA-MB-231 cells were stimulated for 75 minutes with varying concentrations of FVIIa in the presence and absence of FX (175 nM). As controls, cells were stimulated with FX (175 nM) or FXa (175 nM). Induction of IL-8 mRNA was analyzed by Northern blot analysis and quantitated using a PhosphorImager (mean ± SEM, n = 4). NS indicates not statistically significant (P = .3).