Efficiency of expression of FVIII B-domain variants in COS-1 cells is dependent on oligosaccharide content. One-stage activity (□) and FVIII antigen (▪) for FVIII B-domain variant cDNA constructs transiently expressed in COS-1 monkey kidney cells by transfection. One-stage activity was determined by aPTT-based assay. FVIII antigen was determined by a commercial ELISA (Asserachrom FVIII:C Ag). The data were obtained from assaying the conditioned medium at 60 hours following transfection and compared with BDD-FVIII control. Data presented are the mean of several independent experiments (n ≥ 3), and the error bars represent the standard deviation.