Detection of T cells in the peritoneal lavage. The presence of T cells in the peritoneal lavage is detectable IFN-γ–treated male, but not female, mice. Male and female C57/BL6 mice were injected intraperitoneally with 600 U IFN-γ or PBS. Two days later, mice received an intravenous (IV) injection of 107 PKH26-labeled HY-specific T cells. The following day, mice were killed, and the presence of fluorescently labeled cells in the peritoneal lavage (A-B,E-F) and lymph nodes (from IFN-γ–treated mice; C,G) and spleen (from IFN-γ–treated mice; D,H) was assessed by flow cytometry. To facilitate visualization, cells were double stained with a FITC-conjugated anti-CD8 antibody. The mean and SD of the percentage of labeled cells recovered from the peritoneal lavage of at least 3 animals (n) are shown under the relevant panels. *P is significant versus female mice (P < .02).
