Effect of TFPIc23 on cell proliferation. (A) Quiescent HUVECs were stimulated with either bFGF (○) or VEGF (•) in the presence of increasing amounts of the TFPIc23 peptide. BFGF-stimulated HUVECs were also treated with a scrambled TFPIc23 peptide (⋄). Cell proliferation was assessed by quantifying BrdU incorporation in triplicate for each point. Data, presented as percent of growth factor-stimulated HUVECs in the absence of inhibitor, are means ± SD. (B) EOMA hemangioendothelioma (•) and B16 melanoma (○) cells growing in the presence of FBS were treated with increasing amounts of TFPIc23. Cell proliferation was assessed 48 hours later by measuring BrdU incorporation in triplicate for each point. Data are presented as means ± SD. (C) Quiescent HUVECs were stimulated with 10 ng/mL bFGF in the presence of increasing amounts of TFPIc23A (○) and TFPIc23B (•). Cell proliferation was quantified 48 hours later by measuring BrdU incorporation in triplicate for each point. Data are presented as means ± SD. (D) Quiescent human aortic vSMCs were stimulated with 10 ng/mL PDGF, in the presence of increasing amounts of the TFPIc23 peptide. Cell proliferation was measured by cell counts 48 hours later. Data are presented as means ± SD.