Inhibition of in vivo angiogenesis by TFPIc23. (A) Chick CAMs were treated with either PBS or TFPIc23 for 24 hours. TFPIc23 CAMs were uniformly smaller and less well developed than untreated control CAMs. Original magnification × 10. (B) Quantification of the effect of increasing doses of TFPIc23 on chick CAM development. Labeled doses of TFPIc23 were placed on a methyl cellulose disk on top of the CAM. Results are averages ± SD of 4 CAMs/group. (C) Inhibition of in vivo Matrigel plug angiogenesis by TFPIc23. Mice were implanted with Matrigel plugs and treated for 6 days. After treatment, Matrigel plugs were removed and hemoglobin content was measured. Results are average ± SD of 8 plugs.