Decreased expression of CKIϵ in G-CSF–induced granulocytic differentiation of 32D cells. 32D cells were treated with 10 ng/mL G-CSF in a differentiation medium. Total RNAs and cell lysates extracted at days 0, 2, 5, and 10 were subjected to RNA blot analysis by using CKIδ- and CKIϵ-specific cDNA probes (A), or immunoblot analysis using anti-CKIδ, CKIϵ, and β-actin (control) polyclonal antibodies (B). Ethidium bromide (EtBr) staining indicates the amount of RNAs loaded in each lane.