Polymerization profiles of fibrin monomers. (A) Aggregation of fibrin monomers derived from normal fibrinogen (dotted line) or from patient fibrinogen (solid line) at neutral pH in the absence or presence (+Ca²⁺) of 2 mM calcium was monitored at 350 nm. (B) Aggregation profiles of Tokyo V fibrin monomers (solid line; Tokyo V) and those of normal fibrin monomers in the absence (dotted line; normal) or the presence of increasing concentrations (10%, 25%, and 50%) of Tokyo V fibrin monomers (solid line; +Tokyo V) without calcium are shown. +Tokyo V 10%, +Tokyo V 25%, and +Tokyo V 50% indicate that ratios of amounts of normal fibrin monomers and Tokyo V fibrin monomers are 9:1. 3:1, and 1:1, respectively. The concentration of fibrin monomers (the sum of normal fibrin monomers and Tokyo V fibrin monomers) of each preparation was the same. For the control, aggregation profiles of normal fibrin monomers at reduced concentrations without addition of Tokyo V fibrin monomers are shown as dotted lines.