Phenotypic differences between band-3 null strains maintained on different genetic backgrounds. (A) Of inbred band-3 null C3H/HeJ^wan/wan mice, 100% die within 72 hours of birth (○), whereas a targeted strain of band-3 null mice maintained on a hybrid (B6, 129) genetic background show significantly less early mortality, with approximately 15% surviving to adulthood (•). (B) Hematologic analysis of newborn mice obtained using an Advia Multi-species whole-blood analyzer reveals severe anemia in both band-3 null strains, although significant differences are seen. ^*P < .01; ^**P < .001. Error bars indicate standard error.