Efficient lysis of CMV-infected targets by generated CMV-specific T-cell lines. (A) Generated T cells lysed CMV-infected HLA-A^*0201 fibroblasts (▪), whereas noninfected fibroblasts were not lysed (▦; n = 3). (B) Killing of peptide-pulsed T2 cells is shown for 3 donors. T2 cells are loaded with the NLV peptide (▪), the VLE peptide (▦), or an irrelevant MHC class 1 peptide (□), respectively. Displayed data represent chromium release assays performed at an effector/target ratio of 30:1.