Figure 6.
![Figure 6. Loss of alloreactivity after enrichment and expansion of CMV-specific T cells. (A) CD4+ T cells from either the PBMC fraction (d0) or the generated CMV-specific T-cell lines (later than d10) from 3 donors (LSA7, ▦; LSA8, ▪; LSA5, □) were stimulated with irradiated, mature third-party DCs. Proliferative responses are expressed as the median [3H]-thymidine incorporation (cpm) of the stimulated cells - [3H]-thymidine incorporation of the control. (B) CD8+ T cells purified from the PBMC fraction or the generated CMV-specific T-cell lines were plated with irradiated, mature third-party DCs. Numbers of CD8+ T cells were calculated by cell counting.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/103/9/10.1182_blood-2003-09-3056/6/zh80090460760006.jpeg?Expires=1735515685&Signature=bUTG~qMMvrUpiyrT7BJsNWmgpur9CuFQKiYRpy3uVJuUSfjv9bhVxCYaxTGE7gBsec5nRuScrEoK4yL-0spnhvOp~zFDgoHD0TJigCpeNFK0N6yE8LMECrvK6MC6h6bLHsszyEG8or13mOPNpBNSfQF9QmSZ7N6eo6gWcDHHmYNQsB55kj5fDXv045pr5a671H63zeD2ozl0GOXBeaTX5941oZL7np8eKxWd45w6cmDub9XyquXUdEGRkH4NYIpu0aDad1tM46EbrseoEJBfYbXbpolFGCXkAEB2~aVrHaFvRmLRBEACuaUJeUOiUfQxwfsu7WcRovP4nynz4VM25A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Loss of alloreactivity after enrichment and expansion of CMV-specific T cells. (A) CD4+ T cells from either the PBMC fraction (d0) or the generated CMV-specific T-cell lines (later than d10) from 3 donors (LSA7, ▦; LSA8, ▪; LSA5, □) were stimulated with irradiated, mature third-party DCs. Proliferative responses are expressed as the median [3H]-thymidine incorporation (cpm) of the stimulated cells - [3H]-thymidine incorporation of the control. (B) CD8+ T cells purified from the PBMC fraction or the generated CMV-specific T-cell lines were plated with irradiated, mature third-party DCs. Numbers of CD8+ T cells were calculated by cell counting.
