Inhibition of FGFR3 induces cell cycle arrest and is associated with apoptosis of KMS11 and KMS18 cells. (A) KMS11 (top panels) and KMS18 (bottom panels) cells were cultured in the presence of 50 nM PD173074 for the indicated time intervals. Cell cycle profile was evaluated by flow cytometry. The percentage of nuclei in G1 is indicated on each panel. (B) KMS11 (top panel) and KMS18 (bottom panel) cells were cultured with vehicle (▪) or with 12.5 nM (▴)or 50 nM(•) PD173074. Apoptotic cells were identified by annexin V staining and plotted against time. Values are the mean ± SD percentages from triplicate experiments. (C) KMS11 cells were exposed to vehicle (control) or 50 nM PD173074 for 6 days and clot sections of cells were prepared and stained for cleaved caspase 3. Original magnification, × 400.
