Figure 4.
Figure 4. Semiquantitative RT-PCR analysis in patients with NK-LDGL. RT-PCR was performed using primers to amplify the inhibitory receptors KIR2DL1 (2DL1), KIR2DL2 (2DL2), KIR2DL3 (2DL3) and the activating receptors KIR2DS1 (2DS1), KIR2DS2 (2DS2), and KIR2DS4 (2DS4), and β-actin using the conditions described in “Patients, materials, and methods.” Reactions were performed using RNA from 3 healthy individuals and 5 patients with NK-LDGL. The graph represents densitometry results of samples normalized to β-actin and then expressed as a ratio to data obtained from normal 1 that is positive for each reaction. Raw data for normal 1 is shown in Figure 3. Normal 1, normal 2, and normal 3 were run on the same gel with each patient sample and the standard deviation is shown for these samples.

Semiquantitative RT-PCR analysis in patients with NK-LDGL. RT-PCR was performed using primers to amplify the inhibitory receptors KIR2DL1 (2DL1), KIR2DL2 (2DL2), KIR2DL3 (2DL3) and the activating receptors KIR2DS1 (2DS1), KIR2DS2 (2DS2), and KIR2DS4 (2DS4), and β-actin using the conditions described in “Patients, materials, and methods.” Reactions were performed using RNA from 3 healthy individuals and 5 patients with NK-LDGL. The graph represents densitometry results of samples normalized to β-actin and then expressed as a ratio to data obtained from normal 1 that is positive for each reaction. Raw data for normal 1 is shown in Figure 3. Normal 1, normal 2, and normal 3 were run on the same gel with each patient sample and the standard deviation is shown for these samples.

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