Similar KIR surface reactivity on NK and T cells of patients with NK-LDGL. Flow cytometry dot plots of 3-color flow cytometry staining of an NK-LDGL patient (NK-LDGL10). (A) Isotype control-CyChrome (y-axis, left column) and isotype control-FITC (y-axis, right column) and isotype control-PE (x-axis) were used to set the markers. Anti-CD56-CyChrome was used to identify the KIR expression on NK cells and anti-CD8-FITC was used to identify CD8⁺ T cells. CD56+ cells were determined to be CD8^- by examining the expression of CD56-Cychrome versus CD8-FITC. KIRs were identified using the following antibodies: (B) anti-CD158a-PE, (C) anti-CD158b-PE, (D) anti-NKB1-PE, (E) anti-KARp50-PE, and (F) anti-CD94-PE. The percentages of cells positive in each quadrant are indicated in the top right-hand corner of each dot plot. Adding the numbers in the top left and top right quadrants represents the total number of NK cells or T cells (left and right columns, respectively).