Splenocyte expansion following donor pretreatment with standard orpegylated G-CSF. Donor B6 mice (n = 4 per group) received either control diluent, 2 μg human G-CSF/d for 6 days or single injection of 12 μg peg-G-CSF day -6, and splenocytes were harvested on day 7. (A) Relative proportions of each cell lineage. (B) Absolute numbers of each cell lineage. ^*P < .05 control versus peg-G-CSF, ⁺P < .05 peg-G-CSF versus control and G-CSF. Data are presented as mean ± SD.