Glycoprotein expression in platelets from the patient and a control donor as evaluated by flow cytometry. The histograms were obtained using citrated PRP incubated in unstirred suspensions. The MoAbs used against platelet surface glycoproteins were AP-2, directed against αIIbβ3 (A); Bx-1, against GPIbα (C); and Gi9, against α2β1 (D). The activation of αIIbβ3 was evaluated using PAC-1 in whole blood in the presence of 10 μM ADP (B). (E-F) Platelets in PRP were incubated with 50 μM TRAP. In panel E, P-selectin was evaluated using VH10 and, in panel F, granulophysin with MoF11. The light gray lines represent the results for the patient; the heavy lines, the control; and the shaded histograms are those for platelets of the patient incubated without primary antibody.