Figure 6.
FcRγ is present in the patient's platelets, and the ITAM-coupled signaling pathway is functional. (A) Histograms obtained by flow cytometry of permeabilized platelets incubated with a rabbit anti–FcRγ-chain antibody. Results are shown for control platelets (black line), for the patient (gray line) and, for comparison, a negative control performed using the patient's platelets without primary antibody (shaded histogram). (B) Aggregation curves obtained using washed platelets from the patient and a control donor after incubation of platelets with the MoAb IV.3 (anti-FcRγII) followed by F(ab′)2 fragments of a sheep antimouse antibody. Note the positive aggregation response for the patient.
