RT-PCR of mRNA derived from 32D/v, 32D/ICSBP, 32D/BA-v, and 32D/BA-ICSBP-3E cells. As a control, intron-spanning β-actin primers were used. Data from representative experiments are shown. Of note, ICSBP specifically restored the expression of the G-CSFR and C/EBPα, both of which were down-regulated by BCR/ABL. The expression of M-CSFR is specifically induced by ICSBP regardless of the presence of BCR/ABL. Lane 1 indicates 32D/v; lane 2, 32D/ICSBP; lane 3, 32D/BA-v; and lane 4, 32D/BA-ICSBP-3E.