Figure 5.
Effect of CD45-AP expression on CD45 dimerization in ALST-1 cells.Expression of CD45-AP reduces CD45 dimerization in ALST-1 cells. ALST-1 cells stably transfected with HA-CD45-AP or empty vector were biosynthetically labeled with 35S-labeled amino acids. The cell lysates were treated with or without DSP and immunoprecipitated with anti-CD45 antibody. The immunoprecipitates were analyzed by SDS-PAGE under nonreducing conditions. Regions scanned for monomers and dimers are indicated by brackets.
