Platelet protein tyrosine phosphorylation induced by rhodocytin. Platelet-rich plasma from FVB and C57 mice was stimulated with 20 nM rhodocytin for the indicated time points and then solubilized. (A) Total platelet lysates were analyzed by imunoblotting with 4G10 for protein tyrosine phosphorylation (upper panels) and for β-actin (lower panels). (B) Platelet lysates were immunoprecipitated with an antiphospholipase Cγ2 antibody, separated by SDS-PAGE, and imunoblotted with 4G10 for protein tyrosine phosphorylation (upper panels) and for total phospholipase Cγ2 (lower panels). (C) Platelet lysates were immunoprecipitated with an anti-Syk antibody, separated by SDS-PAGE, and imunoblotted with 4G10 for protein tyrosine phosphorylation (upper panels) and for total Syk (lower panels).