Effect of hypoxia on renal eNOS expression and plasma NOx. (A) Representative Western blots showing eNOS antibody detected a single band at the expected 140 kDa; hypoxia increased eNOS staining intensity in both strains of mice. β-actin was used for normalization and verification of protein loading. (B) Changes in plasma nitrite/nitrate after hypoxia. A significant increase in NO_x was detected after 6 hours of hypoxia in Wt mice and in both strains after 12 hours. Values are means ± SEM.