Whole body images of mice taken 7 days after infusion of C57BL/6 BM with or without eGFP Tg donor splenocytes demonstrating more expansion of eGFP Tg splenocytes in the allogeneic setting and discernment of internal organs through the skin of anesthetized mice. (A) Allogeneic B10.BR recipient mouse given B6 BM and eGFP Tg splenocytes. (B) Syngeneic C57BL/6 mouse given C57BL/6 BM and eGFP Tg splenocytes. (C) Allogeneic B10.BR recipient given C57BL/6 BM only (to demonstrate low autofluorescence background). (D) Partial dissection (abdominal membrane still intact) of a mouse similar to that in panel A, showing the view of the lymph nodes from the inner side of the skin. Stereomicroscope was set to 1.0 × zoom factor with a 0.63 × transfer lens.