Figure 4.
Figure 4. eGFP+ cells are present in the lymphoid organs at day 7 after BMT in syngeneic mice, but these cells are either not present or are present only at low frequencies in GVHD target organs compared with allogeneic recipients. Images of close-up views of several organs captured at day 7 after infusion of C57BL/6 BM and eGFP Tg splenocytes in allogeneic and syngeneic recipients as indicated. A cohort of mice receiving an equivalent number of non-eGFP allogeneic splenocytes was also imaged as a control for potential autofluorescence caused by GVHD-induced tissue injury. All images were captured with 1.09-second exposure times to allow direct comparison. The inset shown in the mesenteric node was taken with a 16-msec exposure time because of the intensity of the fluorescence in the nodes. Stereomicroscope was set to 10.0 × zoom factor with a 0.63 × transfer lens. LA indicates long axis cut of head with stereomicroscope set to 3.0 × zoom factor with a 0.63 transfer lens.

eGFP+ cells are present in the lymphoid organs at day 7 after BMT in syngeneic mice, but these cells are either not present or are present only at low frequencies in GVHD target organs compared with allogeneic recipients. Images of close-up views of several organs captured at day 7 after infusion of C57BL/6 BM and eGFP Tg splenocytes in allogeneic and syngeneic recipients as indicated. A cohort of mice receiving an equivalent number of non-eGFP allogeneic splenocytes was also imaged as a control for potential autofluorescence caused by GVHD-induced tissue injury. All images were captured with 1.09-second exposure times to allow direct comparison. The inset shown in the mesenteric node was taken with a 16-msec exposure time because of the intensity of the fluorescence in the nodes. Stereomicroscope was set to 10.0 × zoom factor with a 0.63 × transfer lens. LA indicates long axis cut of head with stereomicroscope set to 3.0 × zoom factor with a 0.63 transfer lens.

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