cFLIP expression in neutrophils. (A) Neutrophils from an SLE patient with neutropenia expressed less cFLIP than from an SLE patient without neutropenia and a healthy volunteer. Representative data from 4 individual experiments. (B) Neutrophils from an SLE patient with neutropenia were incubated with IFN-γ (50 U/mL) or glucocorticoid (500 nM) for various time durations. Glucocorticoid up-regulated cFLIP expression, while IFN-γ slightly down-regulated cFLIP expression in neutrophils. Shown are representative data from 4 individual experiments.