Phenotypic analysis of B cells infected with rF-TRICOM. B cells were infected as described in “Materials and methods.” After 24 hours of culture, B cells were stained with either fluorescein isothiocyanate (FITC)–conjugated antibody (Ab) directed against CD80, or phycoerythrin (PE)–conjugated Ab directed against CD54 and CD58 (shaded histograms). Isotype controls were anti-immunoglobulin (Ig)G1 and anti-IgG2 conjugated to FITC and PE, respectively (open histograms). Analysis was conducted using a FACScan and CellQuest software (BD Biosciences, San Jose, CA). Numbers indicate the percentage of positive cells for each marker; numbers in parentheses indicate MFI (mean fluorescence intensity).