Production of IFN-γ by CD8⁺ T cells isolated from PBMCs from healthy donors after stimulation with Flu peptide-pulsed autologous B cells and DCs. CD8⁺ T cells (1 × 10⁶/mL) were stimulated with irradiated (30 Gy) autologous uninfected B cells (⋄), B cells infected with control vector FP-WT (○), B cells infected with rF-TRICOM (▪), or uninfected DCs from the same donor (▴). (A) Analysis at various Flu peptide concentrations at an APC/effector cell ratio of 1:10. (B) Analysis of rF-TRICOM–infected B cells versus DCs from a different donor at various APC/effector cell ratios when Flu peptide concentration was 0.5 μg/mL. Supernatants were collected and screened for IFN-γ after 24 hours of stimulation. Error bars represent SD of multiple determinations.