Production of IFN-γ by antigen-specific T-cell lines stimulated with allogeneic B cells isolated from PBMCs. (A) CEA-specific V8T cells (0.5 × 106/mL) were stimulated with irradiated allogeneic B cells isolated from PBMCs that were infected with the recombinant fowlpox vectors rF-CEA(6D) or rF-CEA(6D)/TRICOM. As control, B cells were infected with FP-WT. Where indicated, the APCs were pulsed with 25 μg/mL of CAP1-6D peptide. Supernatants were collected after 24 hours of stimulation. (B) MUC-1–specific T cells (0.5 × 106/mL) were stimulated with irradiated allogeneic B cells that were infected with the recombinant fowlpox vectors rF-MUC-1 or rF-MUC-1/TRICOM. As control, B cells were infected with FP-WT. Where indicated, APCs were pulsed with 50 μg/mL of MUC-1 peptide. Supernatants were collected after 48 hours of stimulation. Error bars represent SD of multiple determinations.
