Production of IFN-γ by CEA-specific T cells after activation with peptide-pulsed B cells isolated from PBMCs. CEA-specific V8T cells (0.5 × 10⁶/mL) were stimulated with irradiated allogeneic B cells that were infected with rF-TRICOM (▪) or infected with rF-TRICOM and activated for 24 hours in the presence of 1 μg/mL of CD40L (○) as described in “Materials and methods.” Various concentrations of CAP1-6D peptide were used to pulse the B cells. Supernatants were collected after 24 hours of stimulation. Error bars represent SD of multiple determinations.