Localization of NOS in HMC-1 and LAD 2 cells. HMC-1 or LAD 2 cells were fixed in 4% paraformaldehyde and incubated with rabbit anti-nNOS antibody or rabbit anti-eNOS. Antibody labeling was detected with BODIPY-conjugated goat antirabbit antibodies (green). Nuclei of cells were detected with propidium iodide (red); overlapping fluorescence is indicated in yellow. Original magnification × 600; bar = 10 μm.