Figure 6.
Figure 6. Effect of NOS inhibition and exogenous NO on cysteinyl leukotriene production in human MCs. (A) Concentrations of cysteinyl leukotrienes in medium from HMC-1 cells were determined by ELISA. Cells were preincubated (30 minutes) with the NOS inhibitor (L-NAME), NO donor (SNOG), or cGMP analog (8-bromocGMP), then stimulated with A23187 (1 μM) for 30 minutes. Results are expressed as mean ± SEM for 3 independent experiments. Asterisk indicates P < .01 by comparison with untreated cells. (B) Concentrations of cysteinyl leukotrienes in medium from LAD 2 cells was determined by ELISA. Cells were preincubated (30 minutes) with the NOS inhibitor (L-NAME), NO donor (SNOG), or cGMP analog (8-bromo-cGMP), then stimulated with anti-IgE (1 μg/mL) for 30 minutes. Results are expressed as mean ± SEM for 3 independent experiments. *P < .01 by comparison with untreated cells.

Effect of NOS inhibition and exogenous NO on cysteinyl leukotriene production in human MCs. (A) Concentrations of cysteinyl leukotrienes in medium from HMC-1 cells were determined by ELISA. Cells were preincubated (30 minutes) with the NOS inhibitor (L-NAME), NO donor (SNOG), or cGMP analog (8-bromocGMP), then stimulated with A23187 (1 μM) for 30 minutes. Results are expressed as mean ± SEM for 3 independent experiments. Asterisk indicates P < .01 by comparison with untreated cells. (B) Concentrations of cysteinyl leukotrienes in medium from LAD 2 cells was determined by ELISA. Cells were preincubated (30 minutes) with the NOS inhibitor (L-NAME), NO donor (SNOG), or cGMP analog (8-bromo-cGMP), then stimulated with anti-IgE (1 μg/mL) for 30 minutes. Results are expressed as mean ± SEM for 3 independent experiments. *P < .01 by comparison with untreated cells.

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