Effect of JTZ-132 on mouse (A) and human (B) bone marrow cell differentiation to megakaryocytes. Mouse and human bone marrow cells were cultured in IMDM supplemented with BIT9500 and 2-mercaptoethanol in the presence of JTZ-132 or rhTPO for 6 days and 10 days, respectively. Megakaryocytes were identified after labeling with PE-antimouse CD61 (mouse) and PE-antihuman CD41 (human) monoclonal antibody. Samples were analyzed with FACSort. The values shown are the means of the results from 2 experiments.